Medicinal compounds such as polyamine, sugars, antibiotic, lactams,amino acids, organic acids or redox molecules, play an important role in the human disease prevention and treatment. However, these compounds are not necessary to be chemically synthesized, which is complicated and hard, and sometimes can cause serious environmental pollution.Alternatively, microbial metabolic engineering is an ideal consideration for producing these molecules. In this paper, we preliminarily construct the chemical inducible promoter-biosensor with dynamic genetic regulatory circuit, and assemble the responsive promoters to promote the biosynthesis of putrescine with high efficiency in “push” and “pull” mode. The Cgl1169 and Cgl1141 promoters of Corynebacterium glutamicum (C. glutamicum) that is indeed in responsive to L-Ornithine or putrescine has been screened by immunofluorescence detection (OD535/OD600) and presented good fitting responsive kinetic curve. This new approach can be applied to other promoter screening to construct a fantastic highthroughput platform to large scale screen chemical induced promoters, and to generate drug biosynthesis modular for its production. Thus, these newly fabricated biosensors that utilize the promoter with good properties will not only detect the medical toxic substances, but also can maximum biosynthesis efficiency of medicinal compound, which will further
promote the development of polyamine synthetic microbiology
